A single optical section of the human descending colon collected in vivo following the intravenous administration of 10 ml fluorescein sodium (10 mg/ml; Pharmalab ). The image shows well defined and ordered crypts projecting onto the surface of the colonic mucosa.
The conventional histology image was collected en face from a biopsy section of tissue (6 um thick) following staining with haematoxylin and eosin (H&E; Bar = 100 um). A transverse section of colonic crypts is directly comparable to the en face view of the confocal images. The radial arrangement of the goblet cells and the tall, intervening columnar cells can be observed. Each crypt is surrounded by the connective tissue matrix of the lamina propria.
Single optical section of the human descending colon collected in vivo following the intravenous administration of 10 ml fluorescein sodium (10 mg/ml; Pharmalab). The conventional histology image was collected en face from a biopsy section (6 um thick) following staining with haematoxylin and eosin (H&E). A transverse section of colonic crypts is directly comparable to the en face view of the confocal images. The radial arrangement of the goblet cells and the tall, intervening columnar cells can be observed. Each crypt is surrounded by lamina propria.
Images courtesy of Professor Adrian Polglase, Dr Bill Downey and Dr Wendy McLaren, Cabrini Monash University Department of Surgery, Melbourne, Australia
