CLE imaging in the eye.
Fluorescent labeling: Topical anesthesia was achieved by installation of Minims Amethocaine 1%. Topical fluorescein was applied either by instilling 3 drops of 2% sodium fluorescein solution at 1-minute intervals or by 30 seconds of sustained application of a lightly moistened fluorescein strip to the area of interest.
Confocal Imaging: Confocal endomicroscopy was performed using 488 nm excitation (emission detection >505 nm). The confocal endomicroscope probe was placed in gentle contact with the surface of the eye.