FIVE2 (ViewnVivo)
Handheld confocal in vivo imaging for clinical, translational, preclinical and other research
The hand-held FIVE2 (ViewnVivo) system is the latest generation of miniaturised confocal endomicroscopes. The FIVE2 (ViewnVivo) uses proprietary technology covered by multiple patents, to deliver real-time, cellular level in vivo imaging in living tissue.
Why choose the FIVE2 (ViewnVivo)?
OptiScan specialises in Fluorescence In Vivo Endomicroscopy (FIVE). The flexible hand-held probe of the FIVE2 (ViewnVivo) enables you to push the boundaries of your research with maximum flexibility, viewing image tissue at any angle in 3D with sub-micron resolution.
Superb resolution
Using a single scanned fibre rather than a distally scanned fibre bundle provides unmatched lateral and axial resolution. The single fibre approach eliminates dead spaces and broken fibres, delivering high sensitivity and long life.
Never miss an image
The imaging software of the FIVE2 (ViewnVivo) includes an Image Rollback function. Users can select and save images from up to 60 memory held frames. Individual images or image selections can be saved or made into movie files.
Longitudinal studies
High level disinfection and sterilisation options provide a cost-effective, practical way to enable longitudinal studies on the same subject, as well as the ability to image cell cultures and other samples where sterility is required.
Long-life probes
The FIVE2 (Viewnvivo) probes are not a consumable, but will retain their imaging quality through years of operation and hundreds of procedures.
Precise control in the Z-dimension
The FIVE2 (ViewnVivo) reports the position of the focal plane with micron accuracy. The imaging software provides an intuitive interface to set and collect Z-Stacks. This data can be turned into movies or ported into Fiji for processing.
Image from any angle
Hand held flexible probe lets you access previously inaccessible samples.
FIVE2 (ViewnVivo) features
Compact Processor
The electronics and optics for the system are contained within a single compact box.
Probe Umbilical
Light flexible umbilical for benchtop and stage-mounted applications. Robust chemical resistant umbilical for harsh environments.
Probe Options
A choice of probes to meet different application requirements.
Precision Micro positioner
Small probes can be mounted in the high precision 3 axis micro positioner mounted to an articulated holding arm for maximum flexibility and accuracy in positioning your probe.
3-Pedal Footswitch
Provides hands free control of imaging depth and image capture.
Detachable Probes
Simple one-handed attachment and detachment of probes.
FIVE2 (ViewnVivo) technical specifications
Compared to the previous model, the FIVE2 gives you greater flexibility, increased control and enhanced optimal imaging.
| Features & Specifications | FIVE1 | FIVE2 (ViewnVivo) |
|---|---|---|
Laser power | 10-1000µW | 10-1000µW |
Laser | 488nm | 488nm |
Resolution | 0.7µm lateral 7µm axial | 0.55µm lateral 5.1 µm axial |
Z-depth | 0–250µm | 0–400µm |
Image capture modes | Single frame Continuous capture | Single frame Continuous capture Z-stack Roll-back (60 frames) |
Probes | 150mm X 6.6mmØ 300mm X 6.6mmØ | 46mm X 4.0mmØ * 66mm X 4.0mmØ * 150mm X 4.0mmØ ** 300mm X 4.0mmØ ** |
Umbilical | 2.8M Robust and flexible silicone reinforced with stain-less monocoil and fibreglass braid; 6.5mmØ | *1.5M Light and flexible dual layer mono-coil liner tube with polyolefin jacket; 4.5mmØ **2.8M Robust and flexible silicone reinforced with stain-less monocoil and fibreglass braid; 6.5mmØ |
Sterilisable sheath option | No | Yes |
Filters | 2 Preset filters | 8 Standard filters Long Pass Filters- >515nm, >540nm, >570nm Band Pass Filters- 515-575nm, 515-530nm, 530-550nm, 550-575nm Refection – ND3.0 (0.1% transmission) Additional 4 custom filter positions |
Frame rate | Up to 1.6fps | Up to 3.5fps |
Aspect ratio | 1:1 | 1:1, 5:4, 4:3, 16:10, 16:9 |
Dynamic gain adjustment | No | Yes |
Dynamic filter change | No | Yes |
Probe hot-swap | No | Yes |