Why Confocal Microscopy?
Confocal microscopes make it possible to examine living tissue at high magnification. This is not possible with normal light microscopy as blur and flare from out-of-focus light obscure objects of interest. A confocal microscope overcomes this by using spatial filtering to block out-of-focus light. This results in a high-resolution image that is always in focus.
OptiScan’s laser scanning confocal endomicroscopes are designed for in vivo imaging in medical, pre-clinical and translational applications where conventional confocal microscopes encounter problems accessing the tissue of interest.
The technology platform of the FIVE2 (ViewnVivo) underpins the Zeiss Convivo and is a next-generation advance on the Pentax Medical ISC-1000 and the OptiScan FIVE1.
How it works
OptiScan confocal endomicroscopes use the tip of an optical fibre as a spatial filter, enabling miniaturisation of the microscope.
Blue laser passes through an optical fibre to a flexible hand-held probe. Optics in the probe focus the laser to a spot in the tissue, exciting fluorescence. Emitted light is captured into the optical fibre and passed through an optical filter to a detector. An image is generated by scanning the focussed spot throughout the image plane and compiling the point intensity measurements. The image plane can be translated up and down in the sample, allowing generation of 3D image stacks. For further information, read the answers for some of the frequently asked questions or contact us.
Why single fibre rather than optical fibre bundles?
OptiScan developed the more technically challenging single fibre rather than bundled fibre imaging technology, as it provides the highest possible lateral and axial resolution and enables user-controlled 3D image stack capability, which our users say is of critical importance to imaging in vivo. The merits of this decision have been confirmed in published papers, where the resolution and 3D capability of the FIVE2 (ViewnVivo) probe has been critical to achieving the required diagnostic sensitivity and specificity.